Characterization of mango genotypes of Gir region based on ISSR markers

Abstract

Present experiment was conducted on 20 mango mango genotypes found in Gir region of Gujarat state using 21 ISSR primers. The primers yielded a total 125 bands and 119 scorable polymorphic markers, accounting for 95.2% of total reproducible amplification products in the range 42 to 2522 bp. Each primer could amplify 4 to 11 DNA bands, of which primer UBC-840 generated the highest number of bands (11) followed by UBC 855, UBC 835, and UBC 848. While primers UBC 817, UBC 825, UBC 844, UBC 884 and UBC 891 showed the lowest number (4) of DNA bands. Out of the total 119 polymorphic DNA bands, 34 were unique indicating of its presence only in one of the landraces. Jaccard’s similarity coefficient between genotypes ranged from 0.21 to 0.59. The maximum genetic similarity was found between ‛Jamadar’ and ‘Kesar’ and lowest was between ‘Khodi’ and ‘Agargato’.