In vitro morphogenesis in marigold using shoot tip as explant

Abstract

An effort was made to develop the protocol of in vitro mass multiplication in marigold cultivar Pusa Narangi Gainda from the sterilized selfed seeds on Murashige and Skoog medium supplemented with different combinations of BAP, NAA and GA3. High frequency of germination (84.67%) was obtained 1.0 mg/l BAP and 0.5 mg/l GA3. The growth of individual shoot tip were significantly enhanced (4.1 cm) when the excised shoot tips were transferred on to MS medium with 2.0 mg/l BAP, 0.1 mg/l NAA and 0.5 mg/l GA3. Low auxin (0.2 mg/l NAA) in the culture medium in the contrary to high cytokinin (3.0 mg/l BAP) concentration referred the highest shoot multiplication (10.15 shoots/ explant). Half-strength MS medium supplemented with 1.0 mg/l NAA and 1.0 mg/l IBA was observed to be efficient for rooting (87.33%). The rooted plants could be ideally acclimatized in glasshouse conditions for field transfer.