Diversity analysis in custard apple using RAPD analysis

Abstract

The present investigation was carried out on 12 custard apple genotypes. Isolated and purified DNA was subjected to PCR based marker (RAPD) for assessment of genetic diversity. Out of 15 RAPD primers, eight RAPD primers gave good amplified bands. They gave 32 scorable bands and 130 clear fragments. All 32 bands and fragments were polymorphic with 4.0 polymorphic bands per primer. Primers P-20 and C-04 were the most informative in present investigation. Nei and Li’s dissimilarity coefficient varied from 0.14 to 1.00 and Jaccard’s distance ranged from 0.25 to 1.00, respectively. Binary Squared Euclidean distance was ranged from 3 to 21. The dendrogram was constructed by Ward’s method and clustering of genotypes was also done by using Tocher’s method. Distribution of genotypes was similar in the both methods except two genotypes, i.e., RCA111 and RCA053. The genotype RCA053 had the maximum genetic distance from the other genotypes. There was no relationship between genetic divergence and geographical distance.