RNA isolation from high polyphenol containing tissues of pomegranate

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Published

2015-06-25

Keywords:

Pomegranate, RNA isolation, RNA intergrity number.
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Issue

Vol. 72 No. 02 (2015): Indian Journal of Horticulture

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Articles

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Copyright (c) 2015 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • N.V. Singh ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra
  • Thangasamy Saminathan ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra
  • Ram Chandra ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra
  • Chandrakant Awachare ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra
  • K. Dhinesh Babu ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra
  • D.M. Mundewadikar ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra
  • R.K. Pal ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur 413 255, Maharashtra

Abstract

Three methods of RNA isolation were evaluated based on quality and quantity of the RNA isolated. The methods used for RNA isolation were CTAB-LiCl method, Direct-zol™ RNA MiniPrep (ZymoResearch), and TRIzol® reagent (Life Tech.). RNA quality was assessed using spectrophotometric methods, agarose gel electrophoresis and Agilent 2100 Bioanalyzer. RNA isolation using modified CTAB-LiCl was found effective in extraction of sufficient quantity and high quality RNA from new leaves (617 μg/g FW), seedlings (636 μg/g FW) and flower buds (603 μg/g FW) having RNA integrity number (RIN) values of 8.5 each.

How to Cite

Singh, N., Saminathan, T., Chandra, R., Awachare, C., Babu, K. D., Mundewadikar, D., & Pal, R. (2015). RNA isolation from high polyphenol containing tissues of pomegranate. Indian Journal of Horticulture, 72(02), 273–277. Retrieved from https://www.journal.iahs.org.in/index.php/ijh/article/view/1526

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