Genetic diversity and relationship study of single and double petal tuberose (Polianthes tuberosa L.) cultivars based on RAPD and ISSR markers

Downloads

Published

2016-06-25

Keywords:

Genetic diversity, RAPD, ISSR, tuberose.
Dimensions Badge

Issue

Vol. 73 No. 02 (2016): Indian Journal of Horticulture

Section

Articles

License

Copyright (c) 2016 Indian Journal of Horticulture

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Hemlata Bharti Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012
  • K.P. Singh Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012
  • Rakesh Singh Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012
  • Rajesh Kumar Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012
  • M.C. Singh Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012

Abstract

Tuberose (Polianthes tuberosa L.) is a commercial ornamental bulbous crop with highly fragrant flowers. In the present study, genetic diversity among 12 tuberose cultivars were studied using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Altogether 86 amplicons were amplified with 16 selected RAPD primers out of which, 70 (81.39%) were polymorphic with an average of 4.37 amplicons per primer. Similarly, nine ISSR primers amplified a total of 40 scorable amplicons with sizes ranging between 100-3000 bp. Out of 40 ISSR amplicons, 37 (92.50%) were polymorphic, with an average of 4.1 amplicons per primer. RAPD markers based analysis grouped 12 cultivars into two clusters, whereas ISSR markers based analysis grouped 12 cultivars in three clusters. Analysis of molecular variance (AMOVA) study showed only 5% of variation between single and double petal population, whereas, greater variation (95%) was observed only among the cultivars of each petal type. Principal Coordinate Analysis (PCoA) showed the percentage of variation explained by the first 3 axes was 51.44%.

How to Cite

Bharti, H., Singh, K., Singh, R., Kumar, R., & Singh, M. (2016). Genetic diversity and relationship study of single and double petal tuberose (Polianthes tuberosa L.) cultivars based on RAPD and ISSR markers. Indian Journal of Horticulture, 73(02), 238–244. Retrieved from https://www.journal.iahs.org.in/index.php/ijh/article/view/1580

Downloads

Download data is not yet available.

Similar Articles

<< < 1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 3 > >>