In vitro plant regeneration in brinjal from cultured seedling explants

Abstract

In vitro plant regeneration of brinjal genotype BL-3 was tried using hypocotyl, cotyledon and leaf explants from in vitro raised seedlings on Murashige and Skoog medium fortified with 6-benzylamino purine (BAP) and kinetin (kin) combination (2.0-3.0 mgl-1 BAP with or without 1.0 mgl-1 kin). The cotyledon explant gave cent percent regeneration on MS medium fortified with 2.0 mgl-1 BAP, 2.5 mgl-1 BAP, or 2.5 mgl-1 BAP + 1.0 mgl-1 kin, while the highest numbers of buds on 2.5 mgl-1 BAP (24.90), followed by 2.0 mgl-1 BAP (17.90). Leaf explant also induced cent percent regeneration on MS medium fortified with 2.0 mgl-1 BAP and maximum number of buds (9.53) regenerated with 2.5 mgl-1 BAP. Hypocotyl had the maximum regeneration (66.53%) and maximum buds (3.96) on MS with 2.5 mgl-1 BAP. Maximum bud elongation (58.73%) was obtained on ½ MS medium supplemented with 0.3 mgl-1BAP + double agar. MS basal medium induced maximum rooting of 61.11% plantlets. The hardening with 0.2% bavistin solution enhanced the survival efficiency of plantlets to 81.81%. The plantlets were established in the polythene bags and then transferred to earthen pots in the glasshouse, where they grew, flowered and set fruits.