Molecular characterization of Syzygium cuminii (wild jamun) from A&N Islands

Abstract

Syzygium cuminii (wild jamun) is one of the most important wild fruit tree having great medicinal value. The present investigation was undertaken for characterizing wild jamun genotypes using DNA marker technology. Twenty three genotypes of wild Jamun comprising of twenty one from Andaman and Nicobar Islands and two from mainland were selected for analysis. A set of 30 ISSR and 20 RAPD primers were taken for DNA fingerprinting, among them 17 ISSR and 19 RAPD primers produced 70 and 126 amplicons out of which 43 and 70 amplicons were polymorphic having 61.4 and 55.5% polymorphism, respectively. The maximum discriminating band was obtained from primer ISSR8 and OPA7. Cluster analysis divided the all genotypes into five clusters with both RAPD and ISSR markers. The present study has not only differentiated islands genotypes from mainland but even clustered genotypes separately. The genotype collected from Car Nicobar was distinct with both RAPD and ISSR markers