Effect of plant growth regulators and silver nitrate on female papaya using shoot tip as explant

Abstract

The present study was carried out on Selection 1 cultivar of mature female papaya (Carica papaya L.) by using shoot tip as an explant and media supplementation with the main aim to assess the effect of growth regulators (auxins, cytokinins and silver nitrate) on in vitro regeneration of female papaya. 26 media were used for shoot regeneration while for root regeneration four media were tested supplemented with different concentration of IBA. Based on the results of this study, for shoot proliferation, MS basal medium supplemented with BAP (2.0 mg l-1) + NAA (0.1 mgl-1) was the best followed by MS medium fortified with BAP (2.0 mgl-1) + NAA (0.5 mgl-1) while MS medium supplemented with IBA (2.0 mgl-1) gave best rooting response. Besides, auxins and cytokinins, effect of silver nitrate (AgNO3) on plant regeneration from shoot tip taken from mature female papaya plant was also carried out.