Study on rapid mass multiplication of orchid by using immature seeds

Abstract

Orchid species for present research work used was Bamboo orchid (Arundina graminifolia). The seeds were used as source of ex-plant obtained from immature capsule (120 day old). In vitro culture was initiated on Murashige and Skoog’s medium supplemented with BAP (1.0 mg/l) + NAA (1 mg/l) provided with 16/8 h photoperiod light condition were found to be most suitable for in vitro seed germination and Protocorm Like Bodies (PLB’s) development. The maximum multiplication of the PLB’s was obtained on the MS medium supplemented with, BAP (0.5 mg/l) + NAA (0.5 mg/l) and BAP (1 mg/l) + NAA (1 mg/l) forms plb’s in less period of time i.e. 12.00 and 13 days after inoculation. The MS + BAP (1.5 mg/l) + NAA (1 mg/l) gives highest vegetative growth of plantlets. A well-developed cluster of plantlets were selected and transferred to second subculture for root induction. The half strength MS medium provided with NAA (0.5 mg/l) gives good rooting. Rooted plantlets were transferred in earthen pot with polythene cover filled with peat + soilrite (1:1 v/v) for temporary ex-vitro hardening gives high survival of the seedlings. The plantlets were then successfully acclimatized by additional application of Ohio W. P. solution on same medium using earthen pots.