Quantitative and qualitative analysis of soluble seed protein in okra [Abelmoschus esculentus (L.) Moench]

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Published

2025-12-31

DOI:

https://doi.org/10.58993/ijh/2025.82.4.4

Keywords:

Okra, protein profiling, SDS PAGE, gel electrophoresis, biochemical parameters
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Issue

Vol. 82 No. 04 (2025): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2026 Mukesh Kumar, Prabir Chakraborti

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Mukesh Kumar Department of Seed Science and Technology, Bihar Agricultural University, Sabour (Bhagalpur), Bihar-813210, India
  • Prabir Chakraborti Department of Seed Science and Technology, Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur (Nadia), West Bengal-741 252, India

Abstract

Okra, a warm-season vegetable crop, is a significant source of vitamins, proteins, and minerals. It occupies the fifth position in terms of area and production, with a significant share of okra seed in the domestic and international markets. Seed quality is assessed based on physiological and biochemical parameters, such as protein, carbohydrate, fat, and enzymes. The quantity of seed protein is a strong determinant of seed quality, as it acts as a storage reserve of nitrogen, carbon, and sulphur. Seed protein analysis during development and maturation reveals changes in protein content, composition, and quality. These proteins are crucial for seed viability, growth, and germination. In the present experiment, soluble protein in seeds showed an increasing trend, followed by rapid accumulation at 30 days after anthesis and 35 days after anthesis in pre and post-kharif season respectively. The maximum protein content is recorded at 40 days after anthesis for both growing seasons. Differentiation in banding patterns and developmental stages helps distinguish genotypes. The major difference in banding pattern between 14 kD-45 kD was observed, and developmental stages 25 days after anthesis and 30 days after anthesis were found suitable for genotype differentiation based on SDS-PAGE protein profiling.

How to Cite

Kumar, M., & Chakraborti, P. (2025). Quantitative and qualitative analysis of soluble seed protein in okra [Abelmoschus esculentus (L.) Moench]. Indian Journal of Horticulture, 82(04), 408–414. https://doi.org/10.58993/ijh/2025.82.4.4

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