Biotechnological optimization of In Vitro embryo rescue in citrus: Impact of explant size and cytokinin gradients on germination efficiency
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https://doi.org/10.58993/ijh/2026.83.2.3Keywords:
Embryo rescue, 6-benzylaminopurine (BAP), in vitro morphogenesis, Citrus species, explant physiology.Issue
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Copyright (c) 2026 Shivam .; Amarjeet Kaur
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This study investigated the biochemical impact of 6-benzylaminopurine (BAP) and initial explant size on the in vitro morphogenesis of five Citrus species: C. aurantifolia, C. limon, C. sinensis cv. Mosambi, C. reticulata cv. Kinnow, and C. reticulata Blanco cv. Nagpur mandarin. Mature embryos were cultured on Murashige and Skoog (MS) basal medium integrated with varying cytokinin gradients (0–3 mg/L BAP). Our data reveals a significant species-specific threshold for germination, with C. limon and C. reticulata cv. Kinnow achieving 100% efficiency at an optimal concentration of 2 mg/L BAP. Conversely, concentrations exceeding this limit (3 mg/L) induced an inhibitory effect, likely due to supra-optimal hormonal signaling. Furthermore, a positive correlation was established between explant dimensions and regenerative capacity; larger embryos (7.0–11.0 mm) consistently outperformed smaller cohorts (<5 mm), with C. limon reaching a peak germination rate of 98.46%. These findings provide a refined biotechnological protocol for embryo rescue, essential for overcoming reproductive barriers and accelerating genetic improvement programs in polyembryonic Citrus cultivars.Abstract
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Blanchard, M. L., Barney, J. N., Averill, K. M., Mohler, C. L., & DiTommaso, A. (2010). Does polyembryony confer a competitive advantage to the invasive perennial vine Vincetoxicum rossicum (Apocynaceae)? American Journal of Botany, 97(2), 251-260. https://doi.org/10.3732/ajb.0900232. Bond JE, Roose ML (1998) Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange. Plant Cell Rep 18:229–234 Carvalho, M. P., Santana, D. G., & Ranal, M. A. (2005). Emergência de plântulas de Anacardium humile A. St.-Hil. (Anacardiaceae) avaliada por meio de amostras pequenas. Brazilian Journal of Botany, 28, 627-633. https://doi.org/10.1590/ S0100-84042005000300018 Dutt, M., Vasconcellos, M., & Grosser, J. W. (2010). Effects of various cytokinins on shoot regeneration from leaf explants of commercial Citrus cultivars. Plant Cell, Tissue and Organ Culture, 100, 199–207. FAO (2022). FAOSTAT Statistical Database. Food and Agriculture Organization of the United Nations. Gercheva, P., & Zhivondov, A. (2001). Embryo rescue of early ripening plum cultivars. In VII International Symposium on Plum and Prune Genetics, Breeding and Pomology 577,165-168. DOI: 10.17660/ActaHortic.2002.577.26 Kaur A. (2001). Morphogenesis and regeneration in some citrus species. MSc thesis, GNDU Amritsar. Khan, M. M. A., Mohammad, F., & Siddiqui, M. H. (2019). Plant tissue culture: Applications in crop improvement and conservation. Plant Biotechnology Reports, 13, 421–437. Kishore, K., Monika, N., Rinchen, D., Lepcha, B., & Pandey, B. (2012). Polyembryony and seedling emergence traits in apomictic citrus. Scientia Horticulturae, 138, 101-107. https://doi.org/10.1016/j.scienta.2012.01.035 Liu, W., Chen, X., Liu, G., Liang, Q., He, T., & Feng, J. (2007). Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue. Plant cell, tissue and organ culture, 88, 289-299. https://doi.org/ 10.1007/s11240-007-9201-z Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497 Pérez-Tornero O, Porras I (2008) Assessment of polyembryony in lemon: rescue and in vitro culture of immature embryos. Plant Cell Tissue Organ Cult 93:173–180 Taiz, l.; & Zeiger, E (2004). Fisiologia vegetal. 3.ed. Porto alegre: artmed editora. P.719. Tan ML, Song JK, Deng XX (2007) Production of two mandarin × trifoliate orange hybrid populations via embryo rescue with verification by SSR analysis. Euphytica 157:155–160 Verma, Raj & Singh, Sultan & Sehrawat, SK & Beniwal, VS & Kumar, Mukesh. (2007). In vitro germination of acid lime seed. Haryana Journal of Horticulture Science. 36. 271-272. Viloria Z, Grosser JW, Bracho B (2005) Immature embryo rescue, culture and seedling development of acid citrus fruit derived from interploid hybridization. Plant Cell Tissue Organ Cult 82:159–167 Wakana, A., Ngo, B. X., & Iwamasa, M. (2004). Germinability of embryos during seed development in Citrus (Rutaceae). Journal of the Faculty of Agriculture, 49(1): p049-059 Zhu S, Wu B, Ma Y, Chen J, Zhong G (2013) Obtaining citrus hybrids by in vitro culture of embryos from mature seeds and early identification of hybrid seedlings by allele-specific PCR. Sci Hortic 161:300–305 Zulkarnain, Z., Tapingkae, T., & Taji, A. (2015). Applications of in vitro techniques in plant breeding. Advances in plant breeding strategies: breeding, biotechnology and molecular tools, 293-328. https://doi.org/10.1007/978-3-319-22521-0_10
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