GC-MS based chemical fingerprinting large cardamom cultivars of Sikkim
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https://doi.org/10.58993/ijh/2023.80.4.14Keywords:
Aroma, Large cardamom, 1,8-Cineole, chemical fingerprinting, SikkimIssue
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Copyright (c) 2023 Indian Journal of Horticulture

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Amomum subulatum Roxb, also known as large cardamom, is an herbaceous plant cultivated in Sikkim,Abstract
India for its aromatic seeds with diverse applications in food, beverages, perfumery, and medicine. The study
aimed to GC-MS based chemical fingerprinting through analyzing volatile compounds in seeds and assess
the relatedness among seven large cardamom cultivars grown in the Sikkim hill region. The results revealed
significant differences in volatile compounds among the cultivars. The major compounds identified were
1,8-cineole (76.37-90.63%), α-terpineol (1.73-13.40%), α-terpinolene (0.23-11.51%), β-pinene (0.54-4.13%),
γ-terpinolene (0.77-4.47%), and α-pinene (0.35-1.86%). These compounds possess notable antioxidant, antiinflammatory
and antibacterial properties. Through PCA and dendrogram analyses, it was observed that ICRI
SKM-1 showed similarity to ICRI SKM-2 and Seremna, while Ramsey, Ramla, and Sawney exhibited close
relatedness based on their phytochemical composition. These findings have important implications for large
cardamom breeding programs. These findings also suggest that large cardamom seed extracts could have
potential commercial applications in various industries, including medicine, food, and cosmetics.
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Aftab, A., Singh, V. and Pravej, A. 2020. Morphological and molecular variations studies in high yielding cultivars of Amomum subulatum (Roxb.) collected from north-eastern part of India. Res. J. Biotechnol., 15(10): 80-87. Ali, N.A.A, Wurster, M., Denkert, A., Arnold, N., Fadail, I., Al-Didamony, G., Lindequist, U., Wessjohann, L. and Setzer, W.N. 2012. Chemical composition, antimicrobial, antioxidant and cytotoxic activity of essential oils of Plectranthus cylindraceus and Meriandra benghalensis from Yemen. Nat. Prod. Commun., 7(8): 1099-1102. Boukhatem, M.N., Amine, F.M., Kameli, A., Saidi, F., Walid, K. and Mohamed, S.B. 2014. Quality assessment of the essential oil from Eucalyptus globulus Labill of Blida Debabhuti, N., Mukherjee, S., Neogi, S., Sharma, P., Hossain, U., Maiti, S., Sarkar, M.P., Tudu, B., Bhattacharyya, N. and Bandyopadhyay, R. 2022. A study of vegetable oil modified QCM sensor to detect β -pinene in Indian cardamom. Talanta, 236: 122837. Gurudutt, K.N., Naik, J.P., Srinivas, P. and Ravindranath, B. 1996. Volatile constituents of large cardamom (Amomum subulatum Roxb.). Flavour Fragr. J., 11(1): 7-9. Jabbar, M. and Ghorbaniparvar, H. 2014. Determination of volatile components in black cardamom with gas chromatography-mass spectrometry and chemometric resolution. Int. J. Eng. Res. Technol., 3(11): 1280-1286. Joshi, R., Sharma, P., Sharma, V., Prasad, R., Sud, R.K. and Gulati, A. 2013. Analysis of the essential oil of large cardamom (Amomum subulatum Roxb.) growing in different agro-climatic zones of Himachal Pradesh, India. J. Sci. Food Agric., 93: 1303-1309. kumar, A.K., Vellaikumar, S., Murugan, M., Dhanya, M.K., Ariharasutharsan, G., Aiswarya, S., Akilan, M., Warkentin, T.D. and Karthikeyan, A. 2021. Essential oil profile diversity in cardamom accessions from Southern India. Front. Sustain. Food Syst., 5:1–8. Lee, G.Y., Lee, C., Park, G.H. and Jang, J.H. 2017. Amelioration of Scopolamine-Induced Learning and Memory Impairment by α-Pinene in C57BL/6 Mice. Evid.-Based Complement. Altern. Med. https://doi.org/10.1155/2017/4926815 Li, J., Zhang, C., Gong, M. and Wang, M. 2018. Combination of artemisinin-based natural compounds from Artemisia annua L. for the treatment of malaria: pharmacodynamic and pharmacokinetic studies. Phytother. Res., 32(7): 1415-1420. Mahboubi, M., Heidarytabar, R., Mahdizadeh, E. and Hosseini, H. 2017. Antimicrobial activity and chemical composition of Thymus species and Zataria multiflora essential oils. Agric. Nat. Resour., 51(5): 395-401. Manukyan, A. 2019. Secondary metabolites and their antioxidant capacity of Caucasian endemic thyme (Thymus transcaucasicus Ronn.) as affected by environmental stress. J. Appl. Res. Med. Aromat. Plants, 13. https://doi.org/10.1016/j.jarmap.2019.100209 Mishra, S., Sahu, J.K., Sanwal, N. and Sharma, N. 2021. Hot air convective drying of small cardamom (Elettaria cardamomum Maton): Evaluation of drying, color, and aroma kinetics. J. Food Process. Eng., 44: 1-11. Noumi, E., Snoussi, M., Alreshidi, M.M., Rekha, P.D., Saptami, K., Caputo, L., De Martino, L., Souza, L.F., Msaada, K., Mancini, E., Flamini, G., Al-sieni, A. and De Feo, V. 2018. Chemical and biological evaluation of essential oils from cardamom species. Mol., 23(11): 2818. Shankaracharya, N.B., Naik, P.J., Nagalakshmi, S. and Rao, J.M.L. 1994. Chemical composition and flavour quality of triphal Zanthoxylum rhesta, PAFAI J., 16(1): 15-21 Silva, A.C.R., Lopes, P.M., Azevedo, M.M.B., Costa, D.C.M., Alviano, C.S. and Alviano, D.S. 2012. Biological activities of a-pinene and β-pinene enantiomers. Mol., 17: 6305-16. Silveira, R.D.C., Andrade, L.N. and De Sousa, D.P. 2013. A review on anti-inflammatory activity of monoterpenes. Mol., 18(1): 1227-1254.
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