Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine

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Published

2023-12-30

DOI:

https://doi.org/10.58993/ijh/2023.80.4.2

Keywords:

Banana, Radiation, Mutation, In vitro, LD50
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Vol. 80 No. 04 (2023): Indian Journal of Horticulture

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Articles

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Copyright (c) 2023 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Authors

  • Rajni Rajan Bihar Agricultural University, Sabour 813 210, Bihar, India
  • Muhammad Feza Ahmad Bihar Agricultural University, Sabour 813 210, Bihar, India
  • Awadhesh K. Pal Bihar Agricultural University, Sabour 813 210, Bihar, India
  • Kuldeep Pandey Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh https://orcid.org/0000-0001-7347-5232

Abstract

An investigation was carried out to initiate the in vitro mutagenesis in banana cv. Grand Naine using shoot
tip culture. Based on the sensitivity of explants, the lethal dose was estimated using probit analysis. As a result
of the adverse effects of higher irradiation doses, it was decided that determining the lethal dose can be very
beneficial in reducing population loss. The LD50 dose of gamma irradiation of the banana shoot tip culture was
determined to be 39.5 Gy. The study also discovered that 13 DAS explants when treated with 10 Gy recovered
the highest number of shoots. The optimization of gamma irradiation with subculture days in explants of banana
may be effective in developing significant variations with a particular trait of interest

How to Cite

Rajan, R., Ahmad, M. F., Pal, A. K., & Pandey, K. (2023). Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine. Indian Journal of Horticulture, 80(04), 313–318. https://doi.org/10.58993/ijh/2023.80.4.2

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References

Abdulhafiz, F., Kayat, F. and Zakaria, S. 2018. Effect of gamma irradiation on the morphological and physiological variation from in vitro individual shoot of banana cv. Tanduk (Musa spp.). J. Plant Biotechnol. 45:140-145

Ahloowalia, B.S. 1998. In-vitro techniques and mutagenesis for the improvement of vegetatively propagated plants. In: Brar DS, Ahloowalia BS (ed), Somaclonal variation and induced mutations in crop improvement, pp. 293–309.

Broertjes, C. and Van Harten A.M. 2013. Applied mutation breeding for vegetatively propagated crops. Elsevier, 12.

Elagamawy, M.R. 2002. Radiosensitivity of in vitro Cultured Banana Shoot-Tips. Egypt. J. Radiat. Sci. Appl. 15:133-43.

Ferreira, E.A., Pasqual, M. and Tulmann, N.A. 2009. In vitro sensitivity of fig plantlets to gamma ray. Sci. Agric. 66: 540-542.

Forster, B.P. and Shu, Q.Y. 2012. Plant mutagenesis in crop improvement: basic terms and applications. In: Plant mutation breeding and biotechnology Wallingford, Shu QY, Forster BP, Nakagawa H (eds). CABI, 920 p.

Gomez, K.A. and Gomez, A.A. 1984. Statistical procedures for agricultural research (2nd ed.), John Wiley and Sons, New York, USA. 680 p.

Jain, S.M. 2007. Recent advances in plant tissue culture and mutagenesis. Acta Hort. 736: 205-211

Janick, J. 2008. Plant breeding reviews. John Wiley and Sons. Penjuru, Singapore 30: 61-65

Kaur, S. 2015. Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings (Citrus jambhiri Lush.). J. App. Nat. Sci. 7: 459 – 465.

Kovacs, E. and Keresztes, A. 2002. Effect of gamma and UV-B/C radiation on plant cell. Micron. 33:199-210.

Majeed, A., Khan, A.U.R., Ahmad, H. and Muhammad, Z. 2009. Gamma irradiation effects on some growth parameters of Lepidium sativum L. Am. Eur. J. Agric. Env. Sci. 122: 502–508.

Mishra, P.J., Ganapathi, T.R., Suprasanna, P. and Bapat, V.A. 2007. Effect of single and recurrent gamma irradiation on in vitro shoot cultures of banana. International Journal of Fruit Science. 7: 47-57.

Novak, F.J., Brunner, H. 1992. Plant breeding: induced mutation technology for crop improvement. IAEA Bull. 4: 25-33

Predieri, S. and Zimmerman, R.H. 2001. Pear mutagenesis: In vitro treatment with gamma-rays and field selection for productivity and fruit traits. Euphytica 117: 217–227.

Predieri, S. and Virgilio, N.D. 2007. In vitro Mutagenesis and Mutant Multiplication. In: Jain S.M., Häggman H. (eds) Protocols for Micropropagation of Woody Trees and Fruits. Springer, Dordrecht, pp. 323-333.

Rao, P.S., Ganapathi, T.R., Bapat, V.A., Kulkarni, V. M., Suprasanna, P. 1998. Improvement of banana through biotechnology and mutation breeding. IAEA. 107-118.

Rayan, A.O., Zeinab, A.M., Rekab, A. and Ali, G.A. 2014. In vitro Studies Inducing Genetic Variation in Grape Vine (Vitis vinifera L.) using Gamma Irradiation and Sodium Azide. Middle East J. Agric. Res. 3: 623-630.

Suprasanna, P., Jain, S. M., Ochatt, S. J., Kulkarni, V. M. and Predieri, S. 2012. Applications of in vitro techniques in mutation breeding of vegetatively propagated crops. In: Plant Mutation Breeding and Biotechnology, Shu QY, Forster BP and Nakagawa H. (ed.), CABI Publishing, Wallingford, pp. 371-385.

Yang, P., Li, C., Wei, F., Huang, W., Zheng, J., Huang, D., He, R., Guo, J., Huang, X., Chen, B. and Lin, W. 1995. Radiation mutation induction in ‘China Tianbao’ banana. In: In vitro mutation breeding of bananas and plantains. FAO/IAEA, Vienna, Australia. pp. 17–30.

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