Quantitative and qualitative analysis of soluble seed protein in okra [Abelmoschus esculentus (L.) Moench]

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Published

2025-12-31

DOI:

https://doi.org/10.58993/ijh/2025.82.4.4

Keywords:

Okra, protein profiling, SDS PAGE, gel electrophoresis, biochemical parameters
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Issue

Vol. 82 No. 04 (2025): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2026 Mukesh Kumar, Prabir Chakraborti

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Mukesh Kumar Department of Seed Science and Technology, Bihar Agricultural University, Sabour (Bhagalpur), Bihar-813210, India
  • Prabir Chakraborti Department of Seed Science and Technology, Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur (Nadia), West Bengal-741 252, India

Abstract

Okra, a warm-season vegetable crop, is a significant source of vitamins, proteins, and minerals. It occupies the fifth position in terms of area and production, with a significant share of okra seed in the domestic and international markets. Seed quality is assessed based on physiological and biochemical parameters, such as protein, carbohydrate, fat, and enzymes. The quantity of seed protein is a strong determinant of seed quality, as it acts as a storage reserve of nitrogen, carbon, and sulphur. Seed protein analysis during development and maturation reveals changes in protein content, composition, and quality. These proteins are crucial for seed viability, growth, and germination. In the present experiment, soluble protein in seeds showed an increasing trend, followed by rapid accumulation at 30 days after anthesis and 35 days after anthesis in pre and post-kharif season respectively. The maximum protein content is recorded at 40 days after anthesis for both growing seasons. Differentiation in banding patterns and developmental stages helps distinguish genotypes. The major difference in banding pattern between 14 kD-45 kD was observed, and developmental stages 25 days after anthesis and 30 days after anthesis were found suitable for genotype differentiation based on SDS-PAGE protein profiling.

How to Cite

Kumar, M., & Chakraborti, P. (2025). Quantitative and qualitative analysis of soluble seed protein in okra [Abelmoschus esculentus (L.) Moench]. Indian Journal of Horticulture, 82(04), 408–414. https://doi.org/10.58993/ijh/2025.82.4.4

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References

1. Abdou, A.A. 2006. Identification of some selected and local okra genotypes using the morphological characteristics and protein fingerprinting technique. Al-Azhar J. Agric. Res., 34: 213-227.

2. APEDA 2020. Agricultural and Processed Food Products Export Development Authority, Government of India.

3. Balasubramanian, T. and Sadasivam, S. 1987. Changes in starch, oil, protein and amino acids in developing seeds of okra [Abelmoschus esculentus (L.) Moench]. Plant Foods Hum. Nutr., 37: 41-46.

4. Gustine, D. L., Sherwood, R.T., Gounaris, Y. and Huff, D. 1996. Isozyme, protein and RAPD markers within a half-sib family of buffel grass segregating for Apospory. Crop Sci., 36: 723-727.

5. Hassan, A. E., Heneidak, S. and Gowyad, S. M. H. 2007. Comparative studies of some triticum species by grain protein and amino acid analysis. J. Agron., 6: 286-293.

6. Kamel, E. A., Hassan, H. Z. and Ahmed, S. M. 2003. Electrophoretic characterization and the relationship between some Egyptian cruciferae. J. Biol. Sci., 3: 834-842.

7. Kumar, A., Verma, P. N., Pandey, V. K. and Saha, S. 2024. Genetic variability, correlation, path coefficient analysis and diversity assessment of yield and yield related components in Indian mustard (Brassica juncea L.). Environ. Ecol., 42(4): 1897-1905.

8. NHB 2020. National Horticulture Board, Ministry of Agriculture, Government of India, Website: www.nhb.gov.in.

9. Sadia, M., Salman, A.M., Rabbani, M.A. and Pearce, S.R. 2009. Electrophoretic characterization and the relationship between some Brassica species. Electron. J. Biol., 5: 1-4.

10. Sahoo, M., Sahoo, L., Rai, A. K., Sahoo, A., Mhatre, C., Patra, C. and Phonglosa, A. 2025. Assessment of physiological maturity through pod and seed developmental pattern in local land races of green gram (Vigna radiata L. Wilczek). Int. J. Plant Soil Sci., 37(3): 71-79.

11. Singh, A. and Kaur, A. 2019. Comparative studies on seed protein characteristics in eight lines of two Gossypium species. J. Cotton Res., 2: 6.

12. Singh, A. K., Elango, D., Raigne, J., Van der Laan, L., Rairdin, A., Soregaon, C. and Singh, A. 2025. Plant-based protein crops and their improvement: Current status and future perspectives. Crop Sci., 65(1): e21389.

13. Sridevi R, Manonmani V. 2019. Predicting the optimal stage of maximum seed quality during seed development and maturation in proso millet (Panicum miliaceum L). Int. J. Farm Sci..9(4):89-93

14. Valizadeh, M. 2025. Seed storage protein profile of grain legumes grown in Iran, using SDSPAGE. J. Agric. Sci. Technol., 3(4): 287-292.

15. Vijay, M. K., Tiwari, S., Kahar, L., Malviya, D. and Singh, N. 2025. Fruit and seed development of critically endangered Commiphora wightii (Arn.) Bhandari: Insights into physiological maturation and germination potential. J. Sci. Res. Rep., 31(7): 374-384.

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