Genetic diversity of Ambri apple variants of Jammu region in India
Downloads
Published
Keywords:
Malus × domestica Borkh, cluster analysis, SSR markers,, variabilityIssue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
The distinct germplasm of any crop species constitutes a vital genetic resource for extracting genes or alleles necessary to meet future nutritional and disease-resistance needs. In the present study, a total of 29 SSR markers were used to interpret genetic diversity among fifty Ambri apple variants of the Jammu region, wherein polymorphic information content (PIC), polymorphic percentage, and number of alleles per locus were calculated. Seventeen polymorphic SSR loci amplified 54 alleles, and the number of alleles ranged from 3 to 10, with an average of 5.35 alleles per locus. The mean PIC value for all loci was 0.64. The highest polymorphic percentage (62.50) was observed in primer CH04g10 and the lowest (14.20) in primer CH03d12. Jaccard’s similarity coefficient among Ambri apple variants ranged from 0.14 to 0.74, which indicated a broad genetic base. The cluster dendrogram partitioned the cultivars into two main clusters. Of the 50 collected variants, a wide range of diversity was observed in respect of fruit weight (158.21 to 292.23 g), fruit length (4.35 to 6.37 cm), fruit width (4.62 to 7.62 cm), TSS (13 to 15.91°Brix), titratable acidity (0.30 to 1.7 percent), and ascorbic acid (2.10 to 4.80 mg/100g) content. The highest coefficient of variation was observed in titratable acidity (44.31 percent). Of the selected population, two variants, SKJAD-29 and SKJAD-30, proved promising for commercial cultivation.
How to Cite
Downloads
References
A.O.A.C. 1994. Official Methods of Analysis 16th
Edn. Association of Official Analytical Chemists,
Washington D.C.
A.O.A.C. 1995. Official Methods of Analysis 16th
Edn. Association of Official Analytical Chemists,
Washington D.C.
Ahmed, M. 2008. Biodiversity in pears (Pyrus
sp.): Characterization and conservation of
germplasm from Azad Jammu and Kashmir. Ph.D.
thesis submitted to Department of Horticulture
University College of Agriculture Bahauddin
Zakariya University Multan.
Ahmed, M., Anjum, M.A., Shinwari, K.Z., Awan,
M.S. and Rabbani, M.A. 2011. Assessment of fruit
quality parameters of Pyrus germplasm collected
from Azad Jammu and Kashmir (Pakistan). Pak.
J. Bot.43: 971-81.
Anonymous, 2019. Indian Horticulture Database.
National Horticulture Board. Gurgoan. GOI.
Bostan, S.Z. 2009. Pomological traits of local
apple and pear cultivars and types grown in
Trabzon province. Acta Hortic.825: 293-98.
Cokran, B.D., Karadeiz, T. and Ikten, H. 2019.
Analysis of genetic diversity among ‘Misket’ apple
clones using AFLP, SSR and RAPD markers.
Erwerbs Obstbau.61: 293–302.
Dangl, G. S., Woeste, M. K., Aradhya, M.
K., Koehmstedt, A. and Simon, C. 2005.
Characterization of 14 microsatellite markers
for genetic analysis and cultivar identification of
walnut. J. Am. Soc. Hortic. Sci.130: 348-54.
Doyle, J.J. and Doyle, J.J. 1990. Isolation of plant
DNA from fresh tissue. Focus.12: 13-15.
Farrokhi, J., Darvishzadeh, Naseri. L., Azar, M. M.
and Maleki, H. 2011. Evaluation of genetic diversity
among Iranian apple (Malus × domestica Borkh.)
cultivars and landraces using simple sequence
repeat markers. Aust. J. Crop Sci.5: 815-21.
Galli, Z., Halasz, G., Kiss, E., Heszky, L. and
Dobranszki, J. 2005. Molecularidentification of
commercial apple cultivars with microsatellite
markers. HortSci.40: 1974-77.
Gupta, P. K., Balyan, H.S., Sharma, P.C. and
Ramesh, B. 1996. Microsatellites in plants, a new
class of molecular markers. Curr. Sci.70: 45-54.
Hassan, S., Bhat, K.M. and Rehman, H.U. 2017.
Assessment of Genetic Variability of Wild Apple
(Malus sps) Genotypes in Kashmir Valley. Int. J.
Plant Soil Sci.14: 1-12.
Lavi, U., Cregan, P.B., Schaap, T. and Hillel, J.
Application of DNA markers for identification
and breeding of perennial fruit trees. Plant Breed.
Rev. 7: 195-226.
Misger, F.A. Kumar, A. and Bandey, S.A. 2014.
Performance of some exotic pear cultivars under
temperate condition of Kashmir. J. Hortic. Sci.9:
-47.
Mratinic, E. and Aksic, M.F. 2012. Phenotypic
diversity of apple (Malus sp.) germplasm in South
Serbia. Braz. Arch. Biol. Technol.55: 349-58.
Perrier, X. and Jacquemoud-Collet, J.P. 2006.
DARwin software. http://darwin.cirad.fr/ darwin.
Sharma, R.M., Kour, K., Bandral, J. D., Singh,
B., Rana, J.C. and Jamwal, M. 2016. Analysis
and utilization of genetic diversity of Ambri apple
(Malus × domestica Borkh.) in Jammu region.
Indian J. Hortic.73: 1-7.
